PCR for detection and identification of Streptococcus sobrinus
نویسندگان
چکیده
منابع مشابه
Identification of Streptococcus sobrinus with monoclonal antibodies.
Identification of Streptococcus sobrinus is often difficult to perform because of the great resemblance of the organism to other oral streptococcal species. Therefore, monoclonal antibodies were prepared which were shown to be highly specific for S. sobrinus. Cross-reactivity with other oral microorganisms has not been observed in an enzyme-linked immunosorbent assay and an immunofluorescence a...
متن کاملIdentification and Antibiogram Profile of Streptococcus mutans and Streptococcus sobrinus from Dental Caries Subjects
Article history: Received on: 11/05/2015 Revised on: 29/05/2015 Accepted on: 09/06/2015 Available online: 27/06/2015 Dental caries is one of the oldest disease in the world and its causative agent is mutans streptococci (MS). Among MS, Streptococcus mutans and Streptococcus sobrinus are implicated in caries active subjects. The objective of this study was to identify and determine the antibiogr...
متن کاملBiotyping and Molecular Detection of Streptococcus Mutans and Streptococcus Sobrinus in Caries Active Subjects
Dental caries is one of the most common prevailing disease in humans and a significant public health problem worldwide. Mutans streptococci are a group of oral bacteria, among this group Streptococcus mutans and Streptococcus sobrinus are frequently associated with dental caries. The aim of the present study was (i) To investigate the reliability of morphological, biochemical and multiplex PCR ...
متن کاملDevelopment of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus.
A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.
متن کاملAntibacterial effect of glass ionomer cement and resin cement on cariogenic bacteria of Streptococcus mutans, Lactobacillus acidophilus and Streptococcus sobrinus
Background and Aim: the study of antibacterial properties of resin cements and modified glass ionomers is of great importance. The aim of this study was to determine the antibacterial effect of resin-modified glass ionomers and resin cements on cariogenic bacteria, Streptococcus mutans, Streptococcus sabrinus and Lactobacillus acidophilus. Materials and Methods:In this experimental laboratory s...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2000
ISSN: 0022-2615,1473-5644
DOI: 10.1099/0022-1317-49-12-1069